A Genome-Wide Analysis Indicates that Yeast Pre-mRNA Splicing Is Predominantly Posttranscriptional
نویسندگان
چکیده
منابع مشابه
A genome-wide analysis indicates that yeast pre-mRNA splicing is predominantly posttranscriptional.
Recent ChIP experiments indicate that spliceosome assembly and splicing can occur cotranscriptionally in S. cerevisiae. However, only a few genes have been examined, and all have long second exons. To extend these studies, we analyzed intron-containing genes with different second exon lengths by using ChIP as well as whole-genome tiling arrays (ChIP-CHIP). The data indicate that U1 snRNP recrui...
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as well as inducing conditions. Size analysis, primer Does anyone doubt that Life Sciences—and life—would extension, cDNA cloning, and sequencing indicate that be more dull without genetics? New information often 252 internal nucleotides are missing from the uninduced comes from an expected direction—intellectually as well RNA: a single splicing product, HAC1 mRNA, is present as geographically....
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ژورنال
عنوان ژورنال: Molecular Cell
سال: 2006
ISSN: 1097-2765
DOI: 10.1016/j.molcel.2006.12.002